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We ll talk about a specific method serial dilution, plating, counting, and scaling up, but I want to make it clear at the outset that the problem of godfather 2 gameable content counting huge numbers of things is not at all unique to counting bacteria.
Pull out 1 mL of this latest mixture, and you ll have 1/100th of 1/100th, which is multiplying the fractions together 1/10,000th.
The rest is just technique.And the area of Chicago is about 2100 square miles according to Wikipedia.According to one website, there are 4140 barbers in Chicago in early 2006 - and according to that same website, this is a question sometimes used in job interviews to determine how well candidates think on their feet.Then I put those 3,000 cells into 99 mLs of saline, and took out one mL again, so there must have been 30 cells in that.Why do we dilute.For one thing, the.S.You could literally have a carpet of colonies also known as a confluent lawn growing on your petri dish.Another hint : Multiply by the reciprocal of the dilution factor one more hint : Don t forget that we re only reporting 2 significant digits.
Remember the picture of the hell-to-count petri dish with 500 colonies.
Then we ll end up throwing away 4 of them.
Serial Dilution Introduction: The Purpose of serial dilution is to determine the number of bacteria per unit volume in the origi-nal culture, determination of the.
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